|
| Status |
Public on Jun 19, 2013 |
| Title |
GM_3 |
| Sample type |
RNA |
| |
|
| Source name |
glioblastoma_monolayer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GBM6
cell type: glioma cancer cells
cell subtype: monolayer
|
| Growth protocol |
Monolayer cells were maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum), 100 units/mL penicillin, and 100 μg/mL streptomycin. Spheroid cells were maintained in ultra-low adhesion flasks using serum-free media supplemented with B27, L-Glutamine, Pen/Strep, hEGF (20 ng/ml), and hFGFb (40ng/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using RNeasy mini kit (Qiagen) according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol
|
| |
|
| Hybridization protocol |
Biotinylated cRNA were hybridized to the Illumina array per manufacturer's instructions
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
4621891072_C
|
| Data processing |
Illumina Genome Studio software (v.1.9.0) was used for data processing: background subtraction and Quantile normalization
|
| |
|
| Submission date |
Jun 18, 2013 |
| Last update date |
Jun 19, 2013 |
| Contact name |
Jo Meagan Garner |
| Organization name |
University of Tennessee Health Science Center
|
| Department |
Pathology
|
| Lab |
Pfeffer
|
| Street address |
19 S. Manassas St.
|
| City |
Memphis |
| State/province |
United States |
| ZIP/Postal code |
38103 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE48079 |
Crosstalk between the STAT3, NF-kB and Notch signaling pathways in glioblastoma stem cells |
|
