|
| Status |
Public on Jun 21, 2013 |
| Title |
SSc28lung |
| Sample type |
RNA |
| |
|
| Source name |
SSc-PAH
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SSc-PAH
|
| Data processing |
The data were normalized using median normalization followed by square root transformation in caGEDA [Patel S, Lyons-Weiler J. caGEDA: a web application for the integrated analysis of global gene expression patterns in cancer. Appl Bioinformatics 2004;3(1):49-62]
|
| |
|
| Submission date |
Jun 20, 2013 |
| Last update date |
Jun 21, 2013 |
| Contact name |
Carol Feghali-Bostwick |
| E-mail(s) |
[email protected]
|
| Phone |
412-383-8784
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine
|
| Street address |
MONF 628NW Pittsburgh
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL16221 |
| Series (2) |
| GSE48149 |
Lung tissues in systemic sclerosis have gene expression patterns unique to pulmonary fibrosis and pulmonary hypertension |
| GSE76809 |
Multi-tissue functional genomic study of systemic sclerosis |
|
