|
| Status |
Public on Jul 10, 2013 |
| Title |
Heart_BXD49 ISO |
| Sample type |
RNA |
| |
|
| Source name |
Left Ventricle, 10 wks
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
strain: BXD49/RwwJ
agent: Isoproterenol
tissue: Left Ventricle
|
| Treatment protocol |
Starting at 8-9 weeks, mice were treated for 3 weeks with 30 ug/g body weight/ day of Isoproterenol or were untreated controls for the same period of time
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using the Quiagen miRNAeasy 96-well kits using standard protocol. RNA integrity was determined using an Agilent Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
7754323024_B
|
| Data processing |
GenomeStudio V2010.3 (Gene Expression 1.8.0 module).
Ddata was analyzed using the limma R package.
|
| |
|
| Submission date |
Jul 10, 2013 |
| Last update date |
Jul 10, 2013 |
| Contact name |
Christoph Daniel Rau |
| E-mail(s) |
[email protected]
|
| Organization name |
UCLA
|
| Street address |
675 Charles E Young Drive S
|
| City |
Los Angeles |
| State/province |
United States |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48760 |
Transcriptomes of the hybrid mouse diversity panel subjected to Isoproterenol challenge |
|
