|
| Status |
Public on Jan 31, 2014 |
| Title |
Larval_Brain complexes_wild type_5day AED_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila larval brain complexes day 5
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: wild type
tissue: larval brain complexes
age: 5 d AED
developmental stage: 3rd instar larvae
|
| Treatment protocol |
wing discs or brain complexes were dissected from 3rd instar larvae at day 5 (for wildtype) or day 9 (for mutants) after egg deposition
|
| Growth protocol |
Larvae were incubated at 25oC and grown on standard fly media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were prepared in triplicate, and the RNA isolated using TRIZOL, before being column purified (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standardAffymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was carried out following standard Affymetrix protocols
|
| Scan protocol |
Gene-Chips were washed and stained in the Affymetrix Fluidics Station 400 and scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
no tumours
|
| Data processing |
The data was gcrma normalised using the R Bioconductor package gcrma
|
| |
|
| Submission date |
Jul 14, 2013 |
| Last update date |
Jan 31, 2014 |
| Contact name |
Helena Elizabeth Richardson |
| E-mail(s) |
[email protected]
|
| Phone |
61396561466
|
| Organization name |
Peter MacCallum Cancer Center
|
| Department |
Research Division
|
| Lab |
Cell Cycle and Development lab
|
| Street address |
7 St Andrew's place
|
| City |
East Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3002 |
| Country |
Australia |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE48852 |
Expression profiles from Drosophila melanogaster scrib and lgl mutant wing imaginal disc and brain complexes at day 9 after egg deposition (AED) versus wild type at day 5 AED |
|
