|
| Status |
Public on Jan 01, 2014 |
| Title |
Major_8h_250213 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from NMuMG cells stable transfected with a vector containing Major satellite sequences treated 8 hours with TGFbeta
|
| Organism |
Mus musculus |
| Characteristics |
cell line: NMuMG
treatment: vector with major satellite sequences + TGFbeta
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit QIAGEN
|
| Label |
Biotin
|
| Label protocol |
100-300 ng of total RNA were amplified and labeled using the WT Sense Target labeling system and control reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Sample was hybridized to Mouse Gene 1.0 ST Array (Affymetrix) using a GeneChip Hybridization Oven 646
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 7G
|
| Data processing |
The data were analyzed using the RMA algorithm and then LIMMA was applied to calculate significant differential expression between conditions
|
| |
|
| Submission date |
Jul 22, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Sandra Peiró |
| E-mail(s) |
[email protected]
|
| Phone |
933160434
|
| Organization name |
IMIM-Hospital del Mar
|
| Department |
Programa de Recerca en Càncer
|
| Street address |
Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE49073 |
Gene expression from NMuMG cells overexpressing major satellite treated with TGFbeta |
|
