|
| Status |
Public on Aug 08, 2013 |
| Title |
HUVEC cells from umbilical cords of gestational diabetic mothers GD2 |
| Sample type |
RNA |
| |
|
| Source name |
GESTATIONAL DIABETES
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
ethnicity: Caucasian
condition: gestational diabetes
tissue: umbilical cord
cell type: primary endothelial cells from umbilical cord vein (HUVEC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on custom Affymetrix chips (NuGO_Hs1a520180). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from gestational diabetes samples
|
| Data processing |
The data were normalized with the GCRMA algorithm and analyzed with oneChannelGUI package included in R software
|
| |
|
| Submission date |
Aug 02, 2013 |
| Last update date |
Aug 08, 2013 |
| Contact name |
Guido Leoni |
| E-mail(s) |
[email protected]
|
| Organization name |
Sapienza
|
| Department |
Physics
|
| Street address |
P.le Aldo Moro n5
|
| City |
Rome |
| ZIP/Postal code |
00100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7020 |
| Series (1) |
| GSE49524 |
Differential expression profiles of human primary endothelial cells (HUVECs) from umbilical cords of Gestational Diabetic mothers |
|
