|
| Status |
Public on Aug 19, 2013 |
| Title |
HD A13-CD4+ T-cells |
| Sample type |
RNA |
| |
|
| Source name |
HD CD4+ T-cells
|
| Organism |
Homo sapiens |
| Characteristics |
sample group: healthy donors (HD)
tissue: blood
cell type: CD4+ T-cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from enriched T-cells was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1μg total RNA (GeneChip® Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation and preparation, 10μg of cRNA were hybridized for 16 hr at 45℃ on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
|
| Description |
Gene expression data from enriched global CD4+ T cells from a healthy donor
|
| Data processing |
The data were analyzed with Partek Genomics Suite software (v.6.5) using default analysis settings and normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 18, 2013 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Binbin Zhao |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Pathogen Biology, CAMS&PUMC
|
| Street address |
No. 9 Dong Dan San Tiao, Dongcheng District
|
| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE49954 |
T lymphocytes from Chronic HCV-infected patients express unique pro-apoptotic gene signature. |
|
| Relations |
| Reanalyzed by |
GSE162329 |
