|
| Status |
Public on Sep 07, 2013 |
| Title |
MCF7-2-STR-P60 |
| Sample type |
RNA |
| |
|
| Source name |
Epithelial cells derived from metastatic adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7-STR
gender: Female
age: 69 years
race: Caucasian
|
| Treatment protocol |
Stripped cells were grown over 96 hours (4 successive days) in phenol-red free IMEM media supplemented with 5% Charcoal-Stripped Calf Serum. Every 24 hours the media was removed and replaced with fresh phenol-red free IMEM media supplemented with 5% Charcoal-Stripped Calf Serum (3 times total).
|
| Growth protocol |
Cells were grown in phenol-red free IMEM media supplemented with 5% Charcoal-Stripped Calf Serum. Both Control and Treated cells were kept in a 37C incubator with 5% C02 and regulated humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell lines using TRIzol, cleaned using the QIAGEN Rneasy Mini Kit, and its quality confirmed using an Agilent 2100 Bioanalyzer and RNA 6000 LabChips
|
| Label |
biotin
|
| Label protocol |
Standard one-cycle Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
n/a
|
| Data processing |
The data were analyzed with Affymetrix expression console 1.2.0 using Affymetrix default analysis settings and plier as normalization method.
|
| |
|
| Submission date |
Sep 04, 2013 |
| Last update date |
Sep 07, 2013 |
| Contact name |
Jinghua Gu |
| E-mail(s) |
[email protected]
|
| Organization name |
Virginia Tech
|
| Street address |
900 N.Glebe Rd
|
| City |
Arlington |
| ZIP/Postal code |
22203 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE50564 |
IMPACT: Unraveling intracellular signal transduction and pathway crosstalk by exploring pathway landscape |
|
