|
| Status |
Public on Sep 06, 2013 |
| Title |
2 hours (100 ng/ml FGF2) (2) |
| Sample type |
RNA |
| |
|
| Source name |
Lens primary cultures
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: lens explant
age: 3-day old pups
|
| Treatment protocol |
Rat lens explants were grown overnight in the presence of 5 ng/ml FGF2 (reference sample). The cultures were subsequently treated with 100 ng/ml of recombinant FGF2.
|
| Growth protocol |
Rat lens explants were prepared from 3-day old newborn pups. Six explants were used per individual assay.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
50 ng total RNA was amplified using the NUGEN Ovation™ Biotin RNA Amplification and Labeling System. Amplified cDNA was fragmented and biotin labeled according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
2.2 micrograms of cDNA was hybridized for 17 hr to the Affymetrix Rat Genome 230 2.0 Arrays as described in the Ovation™ Biotin RNA Amplification and Labeling System manual. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station FS 450.
|
| Scan protocol |
Affymetrix GeneArray scanner 7G
|
| Description |
2 hours treated lens extracted total RNA_t2h
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
Sep 05, 2013 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
[email protected]
|
| Phone |
+33648065733
|
| Organization name |
IARC WHO
|
| Street address |
150 COURS ALBERT THOMAS
|
| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE50604 |
Identification and characterization of FGF2-dependent mRNA:microRNA networks during lens fiber cell differentiation |
|
