|
| Status |
Public on Apr 03, 2014 |
| Title |
Fibroblasts, severe phenotype, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
fibroblasts, severe phenotype
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: fibroblast
disease status: recessive dystrophic epidermolysis bullosa (RDEB)
phenotype: severe
|
| Growth protocol |
Primary fibroblast cultures were established from biopsies. Cells were routinely subcultured and used for experiments at passage 6 to 8.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by cultured fibroblasts with TRIzol (Life Technologies).
|
| Label |
Biotin
|
| Label protocol |
cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix scanner.
|
| Description |
FB1636IIpiastra
Gene expression data from cultured fibroblasts from monozygotic twin.
|
| Data processing |
The data were normalized and background corrected using the GCRMA function in R/Bioconductor.
|
| |
|
| Submission date |
Sep 20, 2013 |
| Last update date |
Apr 03, 2014 |
| Contact name |
Paolo Uva |
| Organization name |
IRCCS Istituto Giannina Gaslini
|
| Lab |
Clinical Bioinformatics
|
| Street address |
Via Gerolamo Gaslini, 5
|
| City |
Genoa |
| ZIP/Postal code |
16147 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE51056 |
Monozygotic twins discordant for recessive dystrophic epidermolysis bullosa phenotype highlight the role of TGF-β signalling in modifying disease severity |
|
