Samples were treated with 50nM of PD901, 1000U/mL of interferon beta, or their combination
Growth protocol
All cell lines were plated in 60-80% confluency in RPMI1640, 10%FBS + PenStrep/Glut
Extracted molecule
total RNA
Extraction protocol
Rneasy protocol
Label
Cy3
Label protocol
Agilent's Quick Amp One Color labeling using 100ng total RNA
Hybridization protocol
Agilent's protocol, using 600ng labeled cRNA
Scan protocol
Agilent's protocol
Data processing
Probes were filtered based on their values. Probes with low or high levels in more than 20% of samples were removed. Then samples were normalized based on their 75% percentile
