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Sample GSM1239037 Query DataSets for GSM1239037
Status Public on Jan 01, 2015
Title SkMel133 - Control
Sample type RNA
 
Source name SkMel133 - Control
Organism Homo sapiens
Characteristics cell line: SkMel133
cell type: melanoma
phenotype: low interferon pathway basal activity
Treatment protocol Samples were treated with 50nM of PD901, 1000U/mL of interferon beta, or their combination
Growth protocol All cell lines were plated in 60-80% confluency in RPMI1640, 10%FBS + PenStrep/Glut
Extracted molecule total RNA
Extraction protocol Rneasy protocol
Label Cy3
Label protocol Agilent's Quick Amp One Color labeling using 100ng total RNA
 
Hybridization protocol Agilent's protocol, using 600ng labeled cRNA
Scan protocol Agilent's protocol
Data processing Probes were filtered based on their values. Probes with low or high levels in more than 20% of samples were removed. Then samples were normalized based on their 75% percentile
 
Submission date Sep 24, 2013
Last update date Jan 01, 2015
Contact name Dana Pe'er
Organization name Columbia University
Department Biological Sciences
Lab Dana Pe'er
Street address 1212 Amsterdam Ave.
City New York
State/province NY
ZIP/Postal code 10027
Country USA
 
Platform ID GPL14550
Series (2)
GSE51114 The effect of MEK inhibition, Interferon beta, and their combination on transcription in melanoma
GSE51115 The effect of MEK inhibition on transcription in melanoma

Data table header descriptions
ID_REF
VALUE Log2 normalized intensity levels

Data table
ID_REF VALUE
A_33_P3399172 9.689901
A_33_P3278152 8.076839
A_33_P3361309 14.52633
A_33_P3320718 10.124591
A_24_P350307 14.015976
A_33_P3276329 10.049786
A_33_P3567512 10.923727
A_23_P66563 9.745683
A_33_P3275255 7.688152
A_33_P3351524 8.832682
A_23_P163148 10.511647
A_33_P3351615 14.527833
A_19_P00806158 12.060708
A_24_P101561 9.785538
A_33_P3800664 9.270723
A_33_P3306973 7.165954
A_33_P3407826 10.952067
A_33_P3338961 10.535403
A_19_P00324582 10.279119
A_33_P3701139 10.510725

Total number of rows: 12748

Table truncated, full table size 280 Kbytes.




Supplementary file Size Download File type/resource
GSM1239037_Mel133C.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table
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