|
| Status |
Public on Jan 01, 2015 |
| Title |
SkMel39 - PD901 + Interferon |
| Sample type |
RNA |
| |
|
| Source name |
SkMel39 - PD901 + Interferon
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SkMel39
cell type: melanoma
phenotype: high interferon pathway basal activity
|
| Treatment protocol |
Samples were treated with 50nM of PD901, 1000U/mL of interferon beta, or their combination
|
| Growth protocol |
All cell lines were plated in 60-80% confluency in RPMI1640, 10%FBS + PenStrep/Glut
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy protocol
|
| Label |
Cy3
|
| Label protocol |
Agilent's Quick Amp One Color labeling using 100ng total RNA
|
| |
|
| Hybridization protocol |
Agilent's protocol, using 600ng labeled cRNA
|
| Scan protocol |
Agilent's protocol
|
| Data processing |
Probes were filtered based on their values. Probes with low or high levels in more than 20% of samples were removed. Then samples were normalized based on their 75% percentile
|
| |
|
| Submission date |
Sep 24, 2013 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Dana Pe'er |
| Organization name |
Columbia University
|
| Department |
Biological Sciences
|
| Lab |
Dana Pe'er
|
| Street address |
1212 Amsterdam Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10027 |
| Country |
USA |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE51114 |
The effect of MEK inhibition, Interferon beta, and their combination on transcription in melanoma |
| GSE51115 |
The effect of MEK inhibition on transcription in melanoma |
|
