embryos were collected in 2-hour intervals and aged to generate animals 0-2, 4-6 and 8-10 hour old.
Extracted molecule
total RNA
Extraction protocol
EDTA-treated embryos were lysed in an EDTA extaction buffer (20mM Tris, pH7.4, 140mM KCl, 15mM EDTA, 0.5mM DTT, 1% Triton X-100, 0.1mg/ml cycloheximide, 1mg/ml heparin, 50unit/ml RNasin) and sedimented through 20-50% gradients prepared with the same EDTA lysis buffer, but without Triton X-100.
Label
biotin
Hybridization protocol
Following fragmentation, at least 10 microg of cRNA were hybridized on GeneChip Drosophila Genome 2.0 Array.
Description
Gene expression data from embryos during first 10 hours after egg laying to see the global and transcript-specific translation profile
Data processing
Gene expression measures were computed using the Robust Multichip Average (RMA) method and implemented in the Bioconductor R package.
