|
| Status |
Public on Jan 01, 2014 |
| Title |
6Hz_17_24h__rep_3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Hippocampal cells (sound-stimulated SE mouse NMRI)
|
| Organism |
Mus musculus |
| Characteristics |
time: 24h
replicate: 3
strain: NMRI
cell type: hippocampal cells
|
| Treatment protocol |
After an electrically induced seizure, mice received electrical stimulation (current intensity 44 mA, 0.2 ms monopolar pulses at 6 Hz frequency for 3 s)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the sonicated tissue using miRVANA miRNA isolation kit According to manufacturer instructions
|
| Label |
Cy3
|
| Label protocol |
miRCURY LNATM microRNA Hi-Power Labeling Kit according to manufacturer instructions
|
| |
|
| Channel 2 |
| Source name |
Total RNA from pooled hippocampal cells (sound stimulated SE mouse NMRI)
|
| Organism |
Mus musculus |
| Characteristics |
time: 24h
replicate: 3
strain: NMRI
cell type: hippocampal cells
|
| Treatment protocol |
After an electrically induced seizure, mice received electrical stimulation (current intensity 44 mA, 0.2 ms monopolar pulses at 6 Hz frequency for 3 s)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the sonicated tissue using miRVANA miRNA isolation kit According to manufacturer instructions
|
| Label |
Cy5
|
| Label protocol |
miRCURY LNATM microRNA Hi-Power Labeling Kit according to manufacturer instructions
|
| |
|
| |
| Hybridization protocol |
Tecan hybridization stations with fully automated washing steps according to manufacturer instructions
|
| Scan protocol |
Imagene Scanner according to manufacturer instructions
|
| Description |
6Hz_17_24h
|
| Data processing |
norm exponential convulsion method was used for background correction and loess normalization for within array normalization and quantile normalization for between array normalization, spot quality weights are consided
Series supplementary file normalized.txt includes background-corrected and normalized log2 ratio (Cy5/Cy3)
miRBase 15.0
|
| |
|
| Submission date |
Oct 29, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Khalid A. Abnaof |
| E-mail(s) |
[email protected]
|
| Organization name |
Bonn-Aachen International Centre for Information Technology (B-IT)
|
| Department |
Algorithmic Bioinformatics
|
| Street address |
Dahlmann Straße 2
|
| City |
Bonn |
| State/province |
North Rhine-Westfalina |
| ZIP/Postal code |
53113 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11432 |
| Series (1) |
| GSE51840 |
microRNAs profiling in hippocampal cells of mouse with Sound-induced Seizure |
|
