|
| Status |
Public on Nov 19, 2013 |
| Title |
Islet alpha cell 1 |
| Sample type |
RNA |
| |
|
| Source name |
Islet alpha cell
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed background of B6 and BALB/C
genotype/variation: RIP-CreER; Pdx1fl/+, RosaYFP
cell type: pancreatic islet
cell subtype: normal α-cells
|
| Treatment protocol |
Single-stranded cDNA was generated, labeled, and hybridized with Mouse Gene 1.0 ST Arrays (Affymetrix) and scanned was performed according to the manufacturer's instruction (Affymetrix).
|
| Growth protocol |
RNA was isolated from islet cells with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from islet cells by using an RNeasy total RNA isolation Kit (Qiagen) according to manufacturer’s protocol.
|
| Label |
Biotin
|
| Label protocol |
Five hundred nanograms of total RNA were used for the synthesis of cDNA, followed by amplification and biotin labeling.
|
| |
|
| Hybridization protocol |
A total of 1.5 μg of biotinylated cRNAs was hybridized to the Mouse Gene 1.0ST microarray.
|
| Scan protocol |
After staining with streptavidin-phycoerythrin and biotinylated anti-streptavidin, samples were scanned and data were collected by using GCS3000 laser scanner.
|
| Description |
Alpha1
|
| Data processing |
All statistical analysis was performed using Ingenuity Pathway Analysis database and under the R language environment. A heat map of expression was generated by Treeview.
affymetrix-algorithm-name = rma-gene-default; affymetrix-algorithm-version = 1.0
program-name = Expression Console; program-version = 1.1.2800.19935
|
| |
|
| Submission date |
Nov 11, 2013 |
| Last update date |
Nov 19, 2013 |
| Contact name |
Tao Gao |
| E-mail(s) |
[email protected]
|
| Phone |
215-746-5559
|
| Fax |
215-746-2486
|
| Organization name |
University of Pennsylvania
|
| Street address |
421 Curie Blvd, BRB II/III, Room 527
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE52258 |
Pdx1 maintains β-cell identity and function by repressing an α-cell program |
|
