|
| Status |
Public on Jan 01, 2014 |
| Title |
WT heart, CRE, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Wt Gata4, Wt Gata6, heart, CRE transgenic, biological rep2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: heart
genotype: CRE
age: 8 week adult mice
|
| Treatment protocol |
Cre expression by the MHCalpha transgene leading to Gata4-flox or Gata6-flox gene deletion during late cardiac development.
|
| Growth protocol |
Six-ten week old mice were used for all experiments, and were housed under pathogen-free conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Harvested hearts were homogenized in Trizol and RNA from homogenates was isolated via ethanol precipitation.
|
| Label |
biotin
|
| Label protocol |
Ambion WT labeling kit
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Genes ST 1.0 microarray standard conditions
|
| Scan protocol |
Affymetrix Expression Console
|
| Description |
total heart mRNA gene expression as a function of Gata4 or Gata6 knockout
|
| Data processing |
Expression values were subjected to RMA normalization using Affymetrix CDF probeset definition and GeneSpringGX v7.3.1.
Relative expression levels for each probeset was calculated in reference to the median of its value in Gata4Flox and Gata6Flox non-CRE control samples. These data are on the Series record.
|
| |
|
| Submission date |
Nov 12, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Bruce J Aronow |
| E-mail(s) |
[email protected]
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Biomedical Informatics, Developmental Biology
|
| Lab |
Genome Informatics
|
| Street address |
240 Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE52317 |
Parsing the roles of transcription factors Gata4 and Gata6 in adult cardiac hypertrophic responses |
|
