|
| Status |
Public on Dec 01, 2014 |
| Title |
PXB0282-0049 HCV day10 |
| Sample type |
RNA |
| |
|
| Source name |
HCV day10
|
| Organism |
Homo sapiens |
| Characteristics |
experiment model: human hepatocyte chimeric mice
inoculated with: serum samples from HCV carriers
time: Day 10
tissue: human hepatocytes from mouse liver
|
| Treatment protocol |
Interferon-alpha (7,000IU/g body weight) was administered at week 8 six hours prior to sacrifice in 5 HBV-infected and 5 HCV-infected mice.
|
| Growth protocol |
Mice were inoculated with serum samples from HCV or HBV carriers via the tail vein. Mice were sacrificed by diethyl ether anesthesia. Human hepatocytes from mouse livers were finely dissected and stored in liquid nitrogen after submergence in RNA later solution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using miRNesy mini kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
PXB282-49
HCV-infected mice were euthanized 10 days after infection
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals. Measurements of both duplicate spots with the signal intensities greater than 2 standard deviations (SD) of the background signal intensity were considered to be valid. A relative expression level of a given miRNA was calculated by comparing the signal intensities of the averaged valid spots with their mean value throughout the microarray experiments.
|
| |
|
| Submission date |
Nov 26, 2013 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (2) |
| GSE52752 |
Early and late changes in gene and miRNA expression after hepatitis B or C infection in human hepatocyte chimeric mice [tissue_mRNA] |
| GSE52755 |
Early and late changes in gene and miRNA expression after hepatitis B or C infection in human hepatocyte chimeric mice |
|
