|
| Status |
Public on Dec 31, 2015 |
| Title |
PHH_control_2hr_donor 3 |
| Sample type |
RNA |
| |
|
| Source name |
primary human hepatocytes from donor 3, 0.08% DMSO, 2 hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary human hepatocytes
|
| Treatment protocol |
A 50 mM stock solution of Berberine chloride (Sigma-Aldrich) was prepared in DMSO. Cells were treated with 40 M berberine chloride or 0.08% DMSO as control. Comparing miRNA profiles of 40 M berberine-treated PHHs to those of control cells sampled after 2, 4, and 8 hours treatment
|
| Growth protocol |
The primary human hepatocytes (induction qualified human plateable cryopreserved hepatocytes, HMCPIS) from Caucasian donors were purchased from Invitrogen and cultured following the manufacturer’s instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIZOL reagent (Invitrogen) according to the manufacture’s protocol. The total RNA quantity was measured using a NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies). The total RNA quality and integrity were assayed by Agilent 2100 bioanalyzer with RNA 6000 nano kit (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Agilent's low input quick Amp Labeling Kit according to the manufacture’s protocol
|
| |
|
| Hybridization protocol |
Agilent one color microarray-based gene gexpreesion analysis v4 hybridization protocol
|
| Scan protocol |
Microarrays were scanned following the Agilent microarray scanner protocol, and the image analysis and quantitation were performed by the Agilent Feature Extraction software
|
| Description |
gene expression after 0.08% DMSO treatment for 2hr
|
| Data processing |
The data were normalised using quantile normalization with by GeneSpring GX.
|
| |
|
| Submission date |
Dec 17, 2013 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Ting-fang Lo |
| E-mail(s) |
[email protected]
|
| Phone |
+886-939964794
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Biological Chemistry
|
| Street address |
128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE53415 |
Gene expression profiling of primary human hepatocytes after berberine chloride treatment |
| GSE53416 |
MicroRNA and gene expression profiling of primary human hepatocytes after berberine chloride treatment |
|
