|
| Status |
Public on Dec 01, 2014 |
| Title |
cKO 2h E::1 |
| Sample type |
RNA |
| |
|
| Source name |
uterine sample from cKO female mouse, 2h E2 treated
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: Wnt7aCre+;Esr1f/f (uterine epithelial ERĪ± conditional knockout)
age: 8-10 weeks
gender: female
tissue: uterus
treatment: 17beta-estradiol
time point: 2h
|
| Treatment protocol |
cKO females were injected intraperitoneally with vehicle (saline) or E2 (0.25 ug of 17beta-estradiol) for 2 h or 24 h.
|
| Growth protocol |
cKO females at the age of 8 to 10 weeks were ovariectomized and housed for 10 days before the treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Uterine samples were collected at 2 or 24 h after the injection. Tissues were immediately frozen in liquid nitrogen. RNA was extracted by using RNeasy Mini Kit (Qiagen, Valencia, CA) and treated with DNaseI (Qiagen) according to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Gene expression after 2h E2 treatment in cKO female mouse uterine tissue.
Replicate 1
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v9.5), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Jan 03, 2014 |
| Last update date |
Dec 01, 2014 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE53812 |
Uterine Epithelial Cells Specific Estrogen Receptor alpha-Dependent Gene Expression Profiles in Response to Estrogen |
|
