|
| Status |
Public on Dec 01, 2014 |
| Title |
FAT-M2-b |
| Sample type |
RNA |
| |
|
| Source name |
FAT10 overexpressed mutants with mutation at right region
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colorectal cancer
cell line: HCT116 cells (ATCC)
|
| Growth protocol |
wild type HCT116 cells were grown in McCoy's Dulbecco medium, stable cells were selected and grown in McCoy's Dulbecco medium with Geneticin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate2
|
| Data processing |
The data were normalised using PARTEK software
|
| |
|
| Submission date |
Jan 16, 2014 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Caroline Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
National University of Singapore
|
| Department |
Biochemistry
|
| Lab |
Liver Cancer Functional Genomics
|
| Street address |
14 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE54167 |
Disruption of FAT10-MAD2 binding inhibits tumor progression |
|
