|
| Status |
Public on Sep 22, 2015 |
| Title |
mock infected knockdown flies, 72hpi, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
whole flies mock infected knockdown flies, 72hpi
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: dSTING RNAi
tissue: whole adult flies
pathogen: PBS-control
hours post-infection: 72
|
| Treatment protocol |
Intrathoracic inoculation with a finely pulled glass capillary needle dipped in specified pathogen
Pathogens used for inoculation were Listeria monocytogenes, Drosophila C Virus (DCV), and Insect Iridescent virus 6 (IIV6), at the following concentrations: 1e7 CFU/ml, 5e7 TCID50/ml, 5e6 TCID50/ml. Mock inoculation was performed with PBS. Whole flies were homogenized 24 or 72 h post-infection.
|
| Growth protocol |
F1 generation flies were raised on standard cornmeal and used for all experiments
Parental flies (y1v1;P{TRiP.JF01138}attP2 and y1w*;P{Act5C-GAL4}25FO1/CyO,y+) were mated and 4-7 day old flies were used for all subsequent experiments. dSTING RNAi flies expressed the dsRNA hairpin targeting dSTING and had straight wings, while sibling flies not expressing dSTING dsRNA had curly wings.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Flies were homogenized and total RNA was extracted using Qiagen RNeasy kit. RNA QC was performed using Agilent RNA 6000 Nano chip kit and Agilent Bioanalyzer 2100
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip 3' IVT Express Kit
|
| |
|
| Hybridization protocol |
Affynetrix GeneChip Hybridization, Wash, and Stain Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G Plus
|
| Description |
Gene expression data following inoculation'
dSTING RNAi_mock (PBS)_72h_2
|
| Data processing |
GeneSpring GX 12.0 using the default summarization/scaling/normalization algorithms. Pathogen-inoculated samples were normalized to mock(PBS)-inoculated samples and used for Gene Set Enrichment Analysis
|
| |
|
| Submission date |
Feb 10, 2014 |
| Last update date |
Sep 22, 2015 |
| Contact name |
Glen N. Barber |
| Organization name |
University of Miami
|
| Department |
Cell Biology
|
| Street address |
1550 NW 10th Ave
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE54833 |
Expression data in dSTING knockdown and control sibling flies after mock or pathogenic infection |
| GSE54834 |
Expression data in dSTING knockdown S2 cells and whole flies after mock or pathogenic infection |
|
