|
Status |
Public on Jan 01, 2015 |
Title |
Reduction Mammoplasty [20130313_205635470814_12-20288-A1_06.RCC] |
Sample type |
RNA |
|
|
Source name |
Reduction Mammoplasty
|
Organism |
Homo sapiens |
Characteristics |
age: NA tumor size: NA rfs event: NA rfs time: NA pam50: Normal pam50+claudin-low: Normal ror-s score: 0.313500283934757 ror-s groups: low
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from macrodissected FFPE tissue using ROCHE High Pure FFPE Kit
|
Label |
NA
|
Label protocol |
RNA was not labeled; instead, RNA (100 ng/ul) was pre-processed following the NanoString manufacturer's protocol by VHIO´s Translational Genomics Group
|
|
|
Hybridization protocol |
RNA was hybridized to capture probes and detection probes using the NanoString nCounter system according to the manufacturer's protocol. Detection probes were provided by the manufacturer and were labeled with a proprietary fluorescent barcode system
|
Scan protocol |
Scanning was conducted using the NanoString confocal microscope scanner/Digital Analyzer according to manufacturer's instructions
|
Data processing |
The data were normalized using 5 HK genes. Raw values are the number of copies of a feature-specific molecular barcode that is attached to a detection probe and counted by a confocal system
|
|
|
Submission date |
Feb 20, 2014 |
Last update date |
Jan 01, 2015 |
Contact name |
Aleix Prat |
E-mail(s) |
[email protected]
|
Organization name |
VHIO
|
Department |
Translational Genomics
|
Street address |
Passeig Vall d´Hebron 119
|
City |
Barcelona |
ZIP/Postal code |
08035 |
Country |
Spain |
|
|
Platform ID |
GPL17071 |
Series (1) |
GSE55224 |
A Gene Expression-based Predictor of Fibroepithelial Lesions of the Breast |
|