|
| Status |
Public on Feb 27, 2014 |
| Title |
Neuroretina 8-week lean non-diabetic, Biological replica_4 |
| Sample type |
RNA |
| |
|
| Source name |
Neuroretina 8-week old db/+ mouse: BKS.Cg- (lean)/OlaHsd
|
| Organism |
Mus musculus |
| Characteristics |
genetic background strain: C57BLKS/J
tissue: Neuroretina
age: 8-week old
genotype: db/+
phenotype: lean non-diabetic
|
| Treatment protocol |
Mice were first euthanized by cervical dislocation and then eyes were rapidly removed. Neuroretina was obtained and snap frozen for RNA isolation.
|
| Growth protocol |
8-weeks old db/db diabetic mice and their lean non-diabetic littermates were purchased from Harlan Laboratories.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Rneasy Mini Kit (Qiagen, Germany) according to manufacturer`s instructions.
|
| Label |
Biotin
|
| Label protocol |
200 ng of total RNA were used to synthesize sense ssDNA with the Ambion WT Expression Kit. Next, ssDNA was fragmented and labelled with a biotinylated compound.
|
| |
|
| Hybridization protocol |
Arrays were hybridized for 16 hours at 45° at 60rpm using an Affymetrix hybridization oven.
|
| Scan protocol |
Arrays were scanned on an Affymetrix Scanner 3000 7G
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0). RMA algorithm was used to normalize the arrays and summarized the probes.
|
| |
|
| Submission date |
Feb 26, 2014 |
| Last update date |
Feb 27, 2014 |
| Contact name |
Cristina Hernández |
| E-mail(s) |
[email protected]
|
| Phone |
34 93 489 4172
|
| Organization name |
Vall d'Hebron - Research Institute
|
| Department |
Diabetes and Metabolism
|
| Street address |
Passeig Vall d'Hebron, 119-129
|
| City |
Barcelona |
| ZIP/Postal code |
08035 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE55389 |
Expression data from whole retina of 8-week old db/db diabetic mice and lean littermates |
|
