|
| Status |
Public on Apr 15, 2014 |
| Title |
WT3-3 |
| Sample type |
RNA |
| |
|
| Source name |
WT mESC
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic stem cells 3x passaged without feeder cells
genotype: WT
genetic background: C57BL/6
|
| Growth protocol |
Embryonic stem cell medium was composed of KO-DMEM supplemented with 15% FBS, glutamate, nonessential amino acids, 2-mercaptoethanol (all from Gibco/Life Science Technologies), 103 U/mL LIF (Millipore/GE Healthcare) and 10 mM MgCl2. Cells were grown on the layer of irradiated mouse embryonic fibroblasts. In cultured ES cells, the media was changed daily and cells were split 1:5 every other day. For feeder-free conditions, mESC were depleted of feeder cells by pre-plating on gelatin-coated plates and cultured on 0.1% gelatin-coated plates for 3 passages with 2x103 U/mL LIF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using Qiagen RNAeasy Plus mini Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylation using Illumina¨ TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with Illumina GenomeStudio software
|
| |
|
| Submission date |
Mar 04, 2014 |
| Last update date |
Apr 15, 2014 |
| Contact name |
David E. Clapham |
| E-mail(s) |
[email protected]
|
| Organization name |
HHMI/Boston Children's Hospital/Harvard Medical School
|
| Department |
Cardiology
|
| Street address |
Enders 1309
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE55578 |
Genome-wide comparative analysis of gene expression in wild type (WT3) and TRPM7-/- (K09) mouse embryonic stem cells |
|
