NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM135858 Query DataSets for GSM135858
Status Public on Sep 13, 2006
Title D. simulans pure-species adults, D. simulans pure-species adults, genome chip, replicate 1, Cy3
Sample type RNA
 
Source name Drosophila simulans males, 4 days old
Organism Drosophila simulans
Characteristics Drosophila simulans
Extracted molecule total RNA
Extraction protocol RNA isolation was accomplished with Qiagen RNeasy Mini Kit reagents. The RNA (10 µg) was annealed at the Duke Microarray Core Facility.
Label Cy3
Label protocol The labelling was done at the Duke Microarray Core Facility.
 
Scan protocol Fluorescent array images were collected at the Duke Microarray Core Facility.
Description D. simulans pure-species adults, D. simulans pure-species adults, genome chip
Data processing The median pixel feature intensity after background subtraction (EXPRESSION column) was log2 transformed, and normalized on the median for that hybridization (giving the VALUE column) to obtain the values given in the data table.
 
Submission date Sep 12, 2006
Last update date Jun 12, 2013
Contact name Amanda J. Moehring
E-mail(s) [email protected]
Organization name Duke University
Department DCMB/Biology
Lab Mohamed Noor
Street address Box 91000
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL3829
Series (3)
GSE5601 Comparison of D. simulans/D. mauritiana F1 male hybrid genome-wide expression values to pure-species parents
GSE5607 Comparison of D. simulans/D. sechellia F1 male hybrid genome-wide expression values to pure-species parents
GSE5655 Comparison of pure-species Drosophila expression to hybrid expression

Data table header descriptions
ID_REF clone number for genes with expression in Channel 1
EXPRESSION The channel 2 median pixel feature intensity after background subtraction
VALUE The channel 1 median pixel feature intensity after background subtraction

Data table
ID_REF EXPRESSION VALUE
1773 233 1.069770279
4472 120 0.11247473
14407 2268 4.352789054
604 350 1.656795246
13564 524 2.239007136
3422 120 0.11247473
2669 57 -0.961525852
9292 123 0.148098639
5201 435 1.970455725
11062 93 -0.255257055
1560 187 0.752478594
5089 94 -0.239827014
6766 188 0.760172986
1131 208 0.906023852
4379 57 -0.961525852
11156 116 0.063565129
9798 177 0.673189684
4531 123 0.148098639
8880 4448 5.324525204
11455 74 -0.5849625

Total number of rows: 15552

Table truncated, full table size 320 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap