|
| Status |
Public on May 31, 2014 |
| Title |
Colonic epithelia Muc2-/- mouse 3 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse colonic epithelia (Muc2-/-)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Colonic epithelia
genotype/variation: Muc2-/-
age: 3 months
|
| Treatment protocol |
Isolated colonic epithelial cells were stored at -80 C degree for RNA extraction.
|
| Growth protocol |
Four mice from each genotype group were sacrificed at age of 3 months, and the colonic epithelial cells were isolated using 15 mM EDT buffer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. (Invitrogen)
|
| Label |
FlashTag Biotin HSR
|
| Label protocol |
Total of 200 ng of total RNA were labeled using FlashTag Biotin HSR according prtotocol (Affymatrix: FlashTag™ Biotin HSR RNA Labeling Kit for Affymetrix® GeneChip® miRNA Arrays)
|
| |
|
| Hybridization protocol |
About 130 ul of Affymatrix hybridization cocktail buffer (FS450-002) were used for about 18 hours according to the FS450-002 protocl (Affymatrix).
|
| Scan protocol |
Affymatrix GeneChip Scanner 3000.
|
| Description |
miRNA expression from Muc2-/- mouse colon
|
| Data processing |
Expression Console™ 1.2.0.20
|
| |
|
| Submission date |
Apr 07, 2014 |
| Last update date |
May 31, 2014 |
| Contact name |
Wancai Yang |
| E-mail(s) |
[email protected]
|
| Phone |
3123554154
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Pathology
|
| Street address |
840 South Wood Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE56577 |
Differential expression of microRNA in Muc2-/- mouse colonic epithelial cells |
|
