|
| Status |
Public on May 07, 2014 |
| Title |
CYT1 BioRep1 Techrep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary epithelial Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
expression: ERBB4 expression ICD CYT1
|
| Growth protocol |
Control vector (VEC) and ERBB4 expression MCF10A cells (ICD CYT1 or CYT2) maintained in regular medium containing 10% serum, were collected and RNA samples were extracted and analyzed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Tech Replicate2
|
| Data processing |
Illumina Beadchip Studio, log2 transformed
|
| |
|
| Submission date |
May 06, 2014 |
| Last update date |
May 07, 2014 |
| Contact name |
James T. Platt |
| Organization name |
Yale University
|
| Department |
Medical Oncology
|
| Street address |
333 Cedar ST
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE57339 |
Gene Expression Profiling on Stably Expressing ICD JM-a CYT-1 or CYT-2 ERBB4 isoforms in MCF10A cells |
|
