|
| Status |
Public on Jan 31, 2015 |
| Title |
BreastTumor_OSL2U-0280T1 |
| Sample type |
RNA |
| |
|
| Source name |
Breast primary tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Breast tumor
estrogen receptor (er) status: pos
human epidermal growth factor receptor 2 (her2) status: neg
histological grade: 3
pam50 subgroup: LumB
rppa subgroup: NA
|
| Treatment protocol |
Fresh frozen in liquid nitrogen
|
| Growth protocol |
Human primary tumors
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol, following manufactors protocol
|
| Label |
Cy3
|
| Label protocol |
Agilent miRNA Labeling Reagent and Hybridization Kit, Protocol v.2.3.
|
| |
|
| Hybridization protocol |
Agilent miRNA Labeling Reagent and Hybridization Kit, Protocol v.2.3.
|
| Scan protocol |
Scanning on Agilent scanner G2565A and Feature Extraction (FE) v10.7.3.1 was used to extract signals
|
| Data processing |
Signals were extracted using Feature Extraction v10.7.3.1 and the subsequent data processing was performed using the GeneSpring software v11.0 (Agilent Technologies). In brief, the miRNA signal intensities were log2-transformed and normalized to the 90th percentile, and miRNAs that were detected in less than 10% of the samples were excluded. This resulted in 421 unique mature miRNAs.
|
| |
|
| Submission date |
Jun 04, 2014 |
| Last update date |
Jan 31, 2015 |
| Contact name |
Miriam Ragle Aure |
| E-mail(s) |
[email protected]
|
| Phone |
+47 22781363
|
| Organization name |
Oslo University Hospital Radiumhospitalet
|
| Department |
Dept. of Cancer Genetics
|
| Street address |
Ullernchauséen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10656 |
| Series (4)
|
| GSE58210 |
Integrated analysis reveals microRNA networks coordinately expressed with key proteins in breast cancer [miRNA] |
| GSE58215 |
Integrated analysis reveals microRNA networks coordinately expressed with key proteins in breast cancer |
| GSE81000 |
Integrative clustering reveals a novel split in the luminal A subtype of breast cancer with impact on outcome |
| GSE81002 |
Integrative clustering reveals a novel split in the luminal A subtype of breast cancer with impact on outcome |
|
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