|
| Status |
Public on Jun 09, 2014 |
| Title |
Kras expressing spheres #1 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor spheres at 8 days of culture
|
| Organism |
Mus musculus |
| Characteristics |
tumor: #1
genotype/variation: p48Cre tetO_LKrasG12D ROSA_rtTAL+ p53L+
kras expression: Yes
cell type: Tumor spheres
|
| Treatment protocol |
Doxycycline (Kras expression)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by RNeasy column cleanup was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Tumor spheres derived from the same tumor
|
| Data processing |
Raw data were pre-processed using a robust multi-array analysis
|
| |
|
| Submission date |
Jun 09, 2014 |
| Last update date |
Jun 09, 2014 |
| Contact name |
Sahil Seth |
| E-mail(s) |
[email protected]
|
| Organization name |
MD Anderson
|
| Street address |
1801 East Road
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE58307 |
Expression profiling of KRas ablation surviving cells and matched Kras expressing spheres in pancreatic tumors |
|
