|
| Status |
Public on Aug 01, 2015 |
| Title |
Patient 1 F-1 |
| Sample type |
RNA |
| |
|
| Source name |
Normal fat tissue
|
| Organism |
Homo sapiens |
| Characteristics |
status: n/a
histology: n/a
Sex: M
age: 75
|
| Treatment protocol |
All of the sample were not received preoerative treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from frozen samples using the miRNeasy Mini Kit (Qiagen in Japan, Tokyo, Japan) according to the manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using 3D-Gene Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
The sample was obtained from the case of leiomyosarcoma.
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
Jul 18, 2014 |
| Last update date |
Aug 01, 2015 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE59568 |
Gene expression analysis compared myxoid liposarcoma tissue with normal fat tissue |
|
