|
| Status |
Public on Jun 16, 2015 |
| Title |
Capan-1 cells 24h after siRNA transfection_3 |
| Sample type |
RNA |
| |
|
| Source name |
Capan-1 human pancreatic cancer cell line with TBL1_siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Capan-1
treatment: siRNA-mediated knockdown of TBL1X
|
| Treatment protocol |
Cells were transfected with siRNA against human TBL1X (Qiagen, SI04329514) using Lipofectamine 2000 (Invitrogen) according to manufacturer's instructions
|
| Growth protocol |
Cells were grown in DMEM High Glucose Pyruvate with glutamine (Gibco, #41966) with 10% FBS (Gibco, #10270) and 1% Pen/Strep (Gibco, #15140) and subcultured twice a week by trypsinization
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasy Mini Kit (Qiagen) following manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
gene expression data from Capan-1 cells 24h after siRNA transfection
TBL1_10
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 5, from SAS. Gene expression profiling was performed using arrays of HG-U133_Plus_2 -type from Affymetrix. A Custom CDF Version 14 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Jul 25, 2014 |
| Last update date |
Jun 16, 2015 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16311 |
| Series (1) |
| GSE59761 |
Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy |
|
