|
| Status |
Public on Aug 01, 2007 |
| Title |
siLNA 1 |
| Sample type |
RNA |
| |
|
| Source name |
In vivo siLNA treatment biol. repl. 1
|
| Organism |
Homo sapiens |
| Characteristics |
Human pancreatic xenograft (MiaPaca II)
|
| Treatment protocol |
siLNA treatment via osmotic minipump (0.25 mg/kg/day) for one weeks
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA wre prepared according to standard one cycle target protocol from 10 ug total RNA(Expression Analysis Technical Manual,2001, Affymetrix)
|
| |
|
| Hybridization protocol |
following fragmentation, 10 ug of cRNA wre hybridized for 16 hr at 45C on Human Genome U133plus2 GeneChip. Genechips were washed and stained in a Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips wer scanned using an Affymetrix GC 3000 6G scanner
|
| Description |
Expression data from MiaPaca II tumors treated systhemically with siLNA solution
|
| Data processing |
The data were analyzed as calculated ration profiles with Benjamini-Hochberg FDR correction using Rosetta Resolver.
|
| |
|
| Submission date |
Nov 30, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Olaf Mook |
| E-mail(s) |
[email protected]
|
| Phone |
0031205664540
|
| Organization name |
AMC
|
| Department |
Neurogenetics
|
| Street address |
Meibergdreef 9
|
| City |
Amsterdam |
| ZIP/Postal code |
1105AZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6406 |
Effect on gene expression after administration of siRNA/siLNA targeting GFP |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
