|
| Status |
Public on Apr 01, 2007 |
| Title |
Normal donor_NC04 |
| Sample type |
RNA |
| |
|
| Source name |
CD138 selected bone marrow plasma cells from normal donor.
|
| Organism |
Homo sapiens |
| Characteristics |
Diploid
Normal Chromosome 13
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on HG-U133A GeneChip.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
|
| Description |
Gene expression data from normal donor.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 07, 2006 |
| Last update date |
Mar 29, 2007 |
| Contact name |
Rafael Fonseca |
| E-mail(s) |
[email protected]
|
| Phone |
(480) 301 6363
|
| Fax |
(480) 301 8387
|
| Organization name |
Mayo Clinic
|
| Department |
Hematology-Oncology
|
| Lab |
Hematological Malignancies Laboratory
|
| Street address |
13400 E Shea Blvd
|
| City |
Scottsdale |
| State/province |
AZ |
| ZIP/Postal code |
85260 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6477 |
Expression data from different stages of plasma cell neoplasm |
|
