|
| Status |
Public on Apr 01, 2007 |
| Title |
Human_soft tissue tumor_NCCS085 |
| Sample type |
RNA |
| |
|
| Source name |
Soft tissue tumors - Malignant fibrous histiocytoma
|
| Organism |
Homo sapiens |
| Characteristics |
Gender:female
Histology:Malignant fibrous histiocytoma
Sampling site:primary
|
| Biomaterial provider |
National Cancer Center Hospital, Japan
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumor samples were obtained at the time of surgical excision and cryopreserved in liquid nitrogen until use. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, rev 1, Affymetrix).
|
| |
|
| Hybridization protocol |
Target cRNA preparation from total RNA, hybridization to the microarray, washing and staining with a Single Stain procedure and scanning were all carried out according to the manufacturer’s instructions.
|
| Scan protocol |
GeneChips were scanned using the GeneArray scanner GA2500 (Agilent).
|
| Description |
NCCS085, female, Malignant fibrous histiocytoma
|
| Data processing |
The expression value (Signal) of each probe set was calculated using GeneChip Operating Software (GCOS) ver. 1.3 (Affymetrix), so that the mean of expression values in each experiment was set at 100 to adjust for minor differences between experiments.
|
| |
|
| Submission date |
Dec 07, 2006 |
| Last update date |
Jan 23, 2007 |
| Contact name |
Hitoshi Ichikawa |
| E-mail(s) |
[email protected]
|
| Phone |
+81-3-3542-2511
|
| Fax |
+81-3-3248-1631
|
| Organization name |
National Cancer Center Research Institute
|
| Lab |
Genetics Division
|
| Street address |
5-1-1 Tsukiji, Chuo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6481 |
Gene Expression Analysis of Soft Tissue Sarcomas: Characterization & Reclassification of Malignant Fibrous Histiocytoma |
|
