|
| Status |
Public on Mar 01, 2015 |
| Title |
Male C57BL/6 TCDD 250 μg/kg rep2 |
| Sample type |
RNA |
| |
|
| Source name |
liver_male C57BL/6_TCDD treated_250 μg/kg
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
Sex: Male
age: 12-15 weeks
treated with: 250 μg/kg TCDD
exposure time: 96 hours
tissue: Liver
|
| Treatment protocol |
Animals were treated by oral gavage with a single bolus of either one dosage of TCDD (125, 250, 500, or 1000 μg/kg) dissolved in corn oil vehicle or corn oil alone (10mL/kg).
|
| Growth protocol |
Animals were housed in Macrolon cages with pelleted Altromin 1314 feed and tap water available ab libitum. The housing environment was maintained at 21°C with 50 ± 10% relative humidity and artificial illumination on a 12/12 hour light/dark cycle. Animals were housed individually to avoid aggressive behavior.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy kits according to the manufacturer’s instructions (Qiagen, Mississauga, Canada). Total RNA yield was quantified by UV spectrophotometry and RNA integrity was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
| |
|
| Hybridization protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
| Scan protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
| Description |
HTMoGene11_022312W_PB118_S374_10_RNA.CEL
|
| Data processing |
Raw array data (CEL files) were loaded into the R statistical environment (v3.0.2) using the affy package (v1.28.0) of the BioConductor library. Data was investigated for distributional homogeneity, then pre-processed using the RMA algorithm. An updated Probe to Entrez Gene ID map, mogene11stv1mmentrezgcdf (v15.1.0), was used for annotation.
|
| |
|
| Submission date |
Sep 03, 2014 |
| Last update date |
Mar 01, 2015 |
| Contact name |
Stephenie Prokopec |
| E-mail(s) |
[email protected]
|
| Organization name |
Ontario Institute for Cancer Research
|
| Street address |
661 University Avenue, Suite 510
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17153 |
| Series (1) |
| GSE61038 |
Male and female mice show significant differences in hepatic transcriptomic response to TCDD |
|
