|
| Status |
Public on Sep 23, 2014 |
| Title |
rat hippocampus, high vitamin D, 18 months old, marked, subject 25 |
| Sample type |
RNA |
| |
|
| Source name |
high vitamin D_18 months old_marked
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fisher 344
gender: male
diet group: high vitamin D
tissue: hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from each hippocampal tissue block, as described previously (Blalock et al., 2003; Kadish et al., 2009; Rowe et al., 2007) using TRIzol (Invitrogen) followed by ethanol precipitation, and was reconstituted in RNase-free water (Standard Affymetrix)
|
| Label |
biotin
|
| Label protocol |
For each sample, 20 μg of biotin-labeled cRNA was generated from 5μg of total RNA according to manufacturer’s instructions (Standard Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix- 20μg biotin-labeled cRNA was applied to a Affymetrix Rat Gene 1.0 ST array for hybridization (one chip per animal).
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Hi-10m_080510_(RaGene-1_0-st-v1)
|
| Data processing |
Quality control and signal intensity calculations were performed in Partek (v. 6.6), with additional residual image analysis done in RMAexpress. The gcRMA probe level summary algorithm was used to estimate signal intensity at the gene (rather than exon) level using Partek's default settings.
|
| |
|
| Submission date |
Sep 11, 2014 |
| Last update date |
Sep 23, 2014 |
| Contact name |
Eric M Blalock |
| E-mail(s) |
[email protected]
|
| Phone |
859-323-8033
|
| Organization name |
University of Kentucky
|
| Department |
Molecular and Biomedical Pharmacology
|
| Lab |
Blalock
|
| Street address |
800 Rose St.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40475 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE61326 |
Vitamin D Prevents Cognitive Decline and Enhances Hippocampal Synaptic Function in Aging Rats |
|
