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Sample GSM153085 Query DataSets for GSM153085
Status Public on Jan 16, 2007
Title Tumor samples from patient No.68 PT68U95C
Sample type RNA
 
Source name PT68, tumor samples from patient No.68
Organism Homo sapiens
Characteristics Tissue: primary prostate tumor
Tumor stage: T2b
Gleason Grade: 6
Treatment protocol Fresh prostate tissues were recovered immediately after surgical removal. Tissues were dissected/trimmed to obtain pure tumor then snap frozen in liquid nitrogen within 30 mintues of excision and stored in -80C untill RNA extraction. An H&E stained frozen section of each sample was evaluated by a pathologist, to determine epithelial and stromal content and verify the presence of tumor in the sample. Only tumor tissues with less than 30% of tromal components were selected.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiagen Rneasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U95C array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from primary prostate cancer
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
 
Submission date Dec 22, 2006
Last update date Jan 16, 2007
Contact name Federico Alberto Monzon
E-mail(s) [email protected]
Organization name The Methodist Hospital
Department Pathology
Lab Molecular Diagnostics
Street address 6565 Fannin St, MS205
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL93
Series (2)
GSE6606 Expression data from Primary Prostate Tumor
GSE6919 Expression Data from Normal and Prostate Tumor Tissues

Data table header descriptions
ID_REF 12646
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-MurIL2_at 150.4
AFFX-MurIL10_at 233.9
AFFX-MurIL4_at 66.5
AFFX-MurFAS_at 40.5
AFFX-BioB-5_at 1801.1
AFFX-BioB-M_at 3075.4
AFFX-BioB-3_at 1504.6
AFFX-BioC-5_at 5143.9
AFFX-BioC-3_at 4344.3
AFFX-BioDn-5_at 3666.8
AFFX-BioDn-3_at 22942.3
AFFX-CreX-5_at 41809.3
AFFX-CreX-3_at 66085.1
AFFX-BioB-5_st 63.7
AFFX-BioB-M_st 236
AFFX-BioB-3_st 73.4
AFFX-BioC-5_st 43.3
AFFX-BioC-3_st 46.7
AFFX-BioDn-5_st 406.7
AFFX-BioDn-3_st 614.8

Total number of rows: 12646

Table truncated, full table size 181 Kbytes.




Supplementary file Size Download File type/resource
GSM153085.CEL.gz 2.5 Mb (ftp)(http) CEL

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