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Sample GSM1553

Query DataSets for GSM1553

Status

Public on May 01, 2002

Title

1.3.d-BY

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

[email protected]

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE37

BYxwild_40

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1741	60	1594	62	-0.150684	0
1	549	58	1078	62	1.032325	0
2	1319	55	923	59	-0.565674	0
3	1223	56	817	58	-0.637413	0
4	588	58	550	59	-0.127050	0
5	1402	57	1265	59	-0.174157	0
6	1185	56	1096	59	-0.139410	0
7	1857	56	1793	66	-0.077311	0
8	923	55	1480	64	0.689274	0
9	570	56	816	59	0.541745	0
10	1384	57	1603	61	0.199854	0
11	4762	55	7587	60	0.660487	0
12	6622	57	4203	61	-0.681248	0
13	2677	60	2811	67	0.051586	0
14	1011	70	952	108	-0.173732	0
15	4836	69	5658	124	0.198461	0
16	4992	71	6081	116	0.260790	0
17	2997	71	3519	92	0.211236	0
18	1340	62	1981	72	0.562149	0
19	2856	76	4561	91	0.668410	0

Total number of rows: 7085

Table truncated, full table size 211 Kbytes.

Supplementary data files not provided