|
| Status |
Public on Dec 05, 2014 |
| Title |
Acute Treg, 16 d, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Splenic CD4+CD25+ Treg cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Spleen
cell type: CD4+CD25+ regulatory T cells
infection: 16 days after acute infection with LCMV Arm
|
| Treatment protocol |
Mice were infected with LCMV Arm and LCMV CL13 by intraperitoneal and intravenous injection, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Splenic CD4+CD25+ regulatory T (Treg) cells were isolated from naïve and infected mice at 0 and 16 day after acute and chronic virus infection using a CD4+CD25+ regulatory T Cell Isolation Kit (MiltenyiBiotec). Total RNA was extracted from isolated Treg cells using the RNeasyPlus Micro Kit (Qiagen). RNA purity was confirmed through the Agilent Bioanalyzer 2100 and nano drop 8000 analysis.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (GeneChip® 3’IVT Express kit P/N 901229, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, fragmented cRNA were hybridized for 16 hr at 45C on AffymetrixGeneChip® HT MG-430 PM Array plate. The GeneChips were washed and stained in the Affymetrix GeneTitan® MC Instrument.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® HT Scanner.
|
| Description |
Biological replicate 2 of 3, CD4+CD25+ Treg cells, 16 d after acute infection, isolated from spleen using CD4+CD25+ regulatory T Cell Isolation Kit (MiltenyiBiotec)
|
| Data processing |
The Micorarray Suite version 5 (MAS5) algorithm implemented in Affymetrix Expression Console software (version1.3.1) was used to normalize the raw data. Highly expressed genes that showed over 2-fold difference between the signal values of control and test samples were selected for further study.
|
| |
|
| Submission date |
Dec 04, 2014 |
| Last update date |
Dec 05, 2014 |
| Contact name |
Sang-Jun Ha |
| E-mail(s) |
[email protected]
|
| Phone |
82-10-5552-7683
|
| Organization name |
Yonsei University
|
| Department |
Biochemistry
|
| Lab |
System Immunology
|
| Street address |
50 Yonsei-ro, Science Research Center, Room S423
|
| City |
Seoul |
| ZIP/Postal code |
120-749 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE63876 |
Gene expression profiles of CD4+CD25+ regulatory T cells during acute and chronic viral infection |
|
