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Sample GSM1619

Query DataSets for GSM1619

Status

Public on May 01, 2002

Title

7.3.d-BY

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

[email protected]

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE37

BYxwild_40

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1415	66	2010	81	0.331856	0
1	729	65	1111	79	0.452081	0
2	502	64	790	79	0.514812	0
3	950	63	761	77	-0.559044	0
4	430	61	639	73	0.433075	0
5	847	60	935	73	-0.052782	0
6	192	60	228	73		-50
7	900	58	1537	74	0.612931	0
8	1153	58	1360	77	0.044484	0
9	943	61	1527	80	0.530108	0
10	949	66	1348	85	0.332263	0
11	4372	70	5774	91	0.217539	0
12	6092	73	2595	94	-1.451125	0
13	2302	82	3018	105	0.207839	0
14	1012	88	1034	108	-0.180987	0
15	4291	94	4363	116	-0.167021	0
16	5008	101	5597	129	-0.027935	0
17	3617	118	5333	143	0.384685	0
18	1332	132	2248	166	0.610829	0
19	4916	134	11773	175	1.094083	0

Total number of rows: 7085

Table truncated, full table size 214 Kbytes.

Supplementary data files not provided