|
| Status |
Public on Feb 15, 2007 |
| Title |
Brown Norway Rat Lung- HTV Ventilated_B37 |
| Sample type |
RNA |
| |
|
| Source name |
Rat Lung
|
| Organism |
Rattus norvegicus |
| Characteristics |
Genotype: HTV Ventilated Brown Norway Rats
Weights: 250-350 grams
|
| Treatment protocol |
At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
|
| Growth protocol |
Not Applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Feb 15, 2007 |
| Last update date |
Feb 15, 2007 |
| Contact name |
Ankit A Desai |
| E-mail(s) |
[email protected]
|
| Phone |
312-523-7931
|
| Fax |
773-834-2687
|
| Organization name |
The University of Chicago
|
| Department |
Medicine
|
| Lab |
Joe G.N. Garcia Lab / Center for Integrative Science
|
| Street address |
929 East 57th Street, Room W403R
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE7041 |
Expression data from Rat lungs |
|
