|
| Status |
Public on Oct 10, 2015 |
| Title |
E7.5_Flk-pos Foxf1-pos_sort1 [gene-level] |
| Sample type |
RNA |
| |
|
| Source name |
E7.5 embryo, sorted Flk-pos Foxf1-pos
|
| Organism |
Mus musculus |
| Characteristics |
sorted population: Flk-pos Foxf1-pos
developmental stage: E7.5 embryo
strain: foxf1::venus knock-in, C57BL/6 background
|
| Treatment protocol |
not applicable
|
| Growth protocol |
sorted cells from E7.5 Foxf1::venus Knock-In embryos
|
| Extracted molecule |
total RNA |
| Extraction protocol |
E7.5 embryos were dissociated, stained with antibodies, then 50 cells were sorted by FACS directly into lysis buffer from SuperAmp kit (Miltenyi)
|
| Label |
biotin
|
| Label protocol |
RNA was used to produce biotinylated sense-strand DNA with the Whole Transcript Sense Target Labelling Assay (Affymetrix)
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridised to GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix), following the manufacturer’s instructions
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
0513_VK(9)3_ep_MoEx_Pool3.CEL
|
| Data processing |
Data were processed using Affymetrix Power Tools software package (Affymetrix) for RMA normalization and DABG detection. The expression level of a gene was calculated based on the median expression of its exonic probesets called present. Annotations and cross-mappings between probesets, exons and genes were obtained from Ensembl mouse genome build 70 using the R/BioConductor package annmap.
|
| |
|
| Submission date |
Mar 10, 2015 |
| Last update date |
Oct 10, 2015 |
| Contact name |
Maud Fleury |
| Organization name |
Cancer Research UK Manchester Institute
|
| Lab |
Stem Cell Haematopoiesis
|
| Street address |
Wilmslow Road
|
| City |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19877 |
| Series (1) |
| GSE66574 |
Transcriptome analysis of FOXF1 expressing cells at E7.5 of embryonic development |
|
| Relations |
| Alternative to |
GSM1625691 (exon-level analysis) |
