|
| Status |
Public on Nov 23, 2015 |
| Title |
Activated hepatic stellate cells:L4 |
| Sample type |
RNA |
| |
|
| Source name |
CTC's group: AHSC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Activated hepatic stellate cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from qHSCs, aHSCs and hepatocyte samples using RNeasy MICROKit (QIAGEN GmbH, Hilden, Germany) following a deviation of the standard protocol as recommended by the manufacturer and using 1.5 volumes of 100% ethanol before applying the sample into the column. Total RNA concentration and quality control was assessed using the RNA 6000 pico kit (Agilent, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
| Description |
Activated hepatic stellate cells:L4
sAHSC_L4
|
| Data processing |
Data processing cel files were normalized with 'rma' function of the Bioconductor package (v2.4) in R.3.0.1
|
| |
|
| Submission date |
Apr 08, 2015 |
| Last update date |
Nov 23, 2015 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
[email protected]
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL19099 |
| Series (1) |
| GSE67664 |
Integrative gene expression profiling analysis of human quiescent hepatic stellate cells |
|
