Age: 23m, when sample taken Gender: F Race: Caucasian Illness: Pneumonia/Empyema Treatment: Ceftriaxone Pathogen: S. pneumoniae
Extracted molecule
total RNA
Extraction protocol
RNeasy mini
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol
Standard Affymetrix protocol.
Scan protocol
GeneChips were scanned using the Agilent GeneArray 2500 Scanner.
Description
The subject was infected with S. pneumoniae.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
