|
| Status |
Public on Jan 01, 2008 |
| Title |
B FR13 3 dpi rep 1 with array type rice from Affymetrix |
| Sample type |
RNA |
| |
|
| Source name |
B_FR13_3dpi_rep1
|
| Organism |
Oryza sativa |
| Characteristics |
Oriza sativa - dev.stage (Boyes et al. Plant Cell 2001):last emerged leaf (3d leaf)
|
| Treatment protocol |
Name:infection_rice_pathogen - environmental treatment - pathogen infection,gelatine 0.5% :. Plants were inoculated with Magnaporthe grisea spore suspension as described in Berruyer et al, TAG 2005. Spores were resuspended in gelatine 0.5% at a concentration of 200000 spores/mL. The virulent isolate FR13 was used.
|
| Growth protocol |
leaf - Growth on soil- 60% humidity- 28degreeC day/25degreeC dark- 12h day
|
| Extracted molecule |
total RNA |
| Extraction protocol |
B_FR13_3dpi_rep1:13ug.
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, RNAa 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
Whereas plant disease resistance is well documented at the transcriptomic level, very few analyses have been conducted, especially in cereals, on plant disease susceptibility. In addition, in rice, susceptibility is often not well defined at the phenotypical level and some interactions termed as compatible can be in fact a mixture of compatible and incompatible interactions. We designed a specific experiment in which the interaction between rice and its fungal pathogen Magnaporthe grisea was truly compatible (complete absence of background resisatnce) in order to identify genes that would be differentially regulated in such condition. These genes may potentially represent targets for effectors of the pathogen.
|
| Data processing |
Normalized data by GCRMA
|
| |
|
| Submission date |
Mar 13, 2007 |
| Last update date |
Mar 13, 2007 |
| Contact name |
sandrine balzergue |
| Organization name |
INRA
|
| Lab |
IRHS
|
| Street address |
Rue G. Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49000 |
| Country |
France |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE7256 |
Identification of rice genes differentially expressed upon virulent infection by Magnaporthe grisea |
|
