|
| Status |
Public on Oct 30, 2007 |
| Title |
Female lung, uninfected (rep3) |
| Sample type |
RNA |
| |
|
| Source name |
Female lung, uninfected (rep3)
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: uninfected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
|
| Label |
biotin
|
| Label protocol |
7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
|
| |
|
| Hybridization protocol |
10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
|
| Description |
sham = gonadally intact
|
| Data processing |
The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
|
| |
|
| Submission date |
Mar 14, 2007 |
| Last update date |
Oct 30, 2007 |
| Contact name |
Sabra Lynn Klein |
| E-mail(s) |
[email protected]
|
| Phone |
(410) 955-8898
|
| Fax |
(410) 955-0105
|
| Organization name |
Johns Hopkins Bloomberg School of Public Health
|
| Department |
Molecular Microbiology and Immunology
|
| Lab |
Lab of Sabra Klein
|
| Street address |
615 North Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE7271 |
Sex differences in gene expression profiles during hantavirus infection of rats |
|
