|
| Status |
Public on Apr 11, 2016 |
| Title |
Gfi1_WT_Th |
| Sample type |
RNA |
| |
|
| Source name |
splenic naïve CD4 T cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Gfi1 wild type
tissue: spleen
|
| Growth protocol |
Naïve CD4 T (CD44lowCD62LhighCD25negative) cells were prepared using a CD4+CD62L+ T cell isolation kit II (cat#130-093-227; Miltenyi Biotec). Naïve CD4 T cells were stimulated with an immobilized anti-TCR-β mAb (3 μg/ml, H57-597; BioLegend), an anti-CD28 mAb (1 μg/ml, 37.5; BioLegend), IL-2 (10 ng/ml), anti-IL-4 mAb (5μg/ml, 11B11; BioLegend), and anti-IFN-γ mAb (5 μg/ml, R4-6A2; BioLegend) for two days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with PureLink RNA Mini Kit (lifetechnologies) according to the manufacture's protocol
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA was amplifies and labeled using Low Input Quick Amp Labeling Kit, One-Color
|
| |
|
| Hybridization protocol |
Hybridization and washing were done according to Agilent One-Color Gene Expression protocol
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent SureScan microarray scanner (G2600D) using one color scan setting
|
| Description |
gene expression in Gfi1_WT_Thn
|
| Data processing |
Raw signal intensities were extracted with Agilent Feature Extraction Software 11.5.1.1 (protocol GE1_1105_Oct12) using default parameters
|
| |
|
| Submission date |
Jul 13, 2015 |
| Last update date |
Apr 11, 2016 |
| Contact name |
Junpei Suzuki |
| Organization name |
Ehime University
|
| Department |
Graduate School of Medicine
|
| Lab |
Immunology
|
| Street address |
Shitukawa
|
| City |
Toon |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13912 |
| Series (2) |
| GSE70829 |
Gfi1, a transcriptional repressor, inhibits the induction of the T helper type 1 programme in activated CD4 T cells |
| GSE70894 |
The transcriptional repressor Gfi1 inhibits the induction of Th1 program in activated CD4 T cells |
|
