|
| Status |
Public on Jul 29, 2015 |
| Title |
OAT_Obese non-diabetic [Lot OM032608B] |
| Sample type |
RNA |
| |
|
| Source name |
Omental adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Omental adipose tissue samples were disrupted and homogenized in QIAzol lysis reagent using a tissuelyser (Qiagen Inc., Valencia, CA) and followed by chloroform extraction. The upper aqueous phase containing the RNA was processed with EZ1 RNA universal tissue kit on the EZ1 workstation following manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Per RNA labeling, 500 ng of total RNA was used in conjunction with the Illumina® TotalPrep RNA Amplification Protocol and labelled biotin.
|
| |
|
| Hybridization protocol |
Labeled cRNAs was hybridized to the Affymetrix Human Genome U133 2.0 Gene Chip according to manufacturer's protocol.
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays
|
| Description |
gene expression data from omental adipose tissue of obese and non-diabetic African American
|
| Data processing |
Probe cell intensity data (Affymetrix CEL files) were generated using Affymetrix GeneChip Command Console (AGCC) software. Affymetrix microarray CEL files with probe cell information were further processed in Partek Genomics Suite 6.0 (St Louis, MO) and were summarized into probe-set level data with robust multi-array average (RMA) algorithm
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
Jul 29, 2015 |
| Contact name |
Ayo P Doumatey |
| Organization name |
NIH
|
| Department |
NHGRI
|
| Lab |
CRGGH
|
| Street address |
12 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE71416 |
Gene Expression Profiling in Omental Adipose Tissue of Morbidly Obese Diabetic African Americans |
|
