|
| Status |
Public on Mar 16, 2016 |
| Title |
pES10 EB d-G1 |
| Sample type |
SRA |
| |
|
| Source name |
Diploid pES10-EB cells in G1 (2c)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: d-pES10
cell type: Diploid pES10-EB cells
cell cycle phase: G1 (2c)
passage: 12
differentiation status: Differentiated
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Micro Kit or the RNeasy Mini Kit (Qiagen). For RNA sequencing analysis, poly(A) pull-down was used to enrich mRNAs from total RNA samples (200 ng–1 µg per sample)
Libraries were prepared using the Illumina TruSeq RNA prep kit v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Diploid EB cells in G1 (2c) derived from ES cells after 21 days differentiation
|
| Data processing |
Reads were mapped to the human reference genome (GRCh37/hg19) using TopHat (version 2.0.8b)
Reads per kilobase of transcript per million mapped reads (RPKM) values were calculated using Cufflinks (version 2.1.1)
Genome_build: Ensembl/GRCh37/hg19
Supplementary_files_format_and_content: Tab-delimited text file includes Abundance measurements (RPKM) for each sample
|
| |
|
| Submission date |
Jul 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ido Sagi |
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
|
| Street address |
Givat Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE71456 |
Derivation and differentiation of haploid human embryonic stem cells [RNA-Seq 1] |
| GSE71458 |
Derivation and differentiation of haploid human embryonic stem cells |
|
| Relations |
| BioSample |
SAMN03940964 |
| SRA |
SRX1122439 |
